P-111: Inhibitory Effect of Noscapine on Human Endometriotic Epithelial Cells

Background: Endometriosis is the presence of a functional endometrial tissue outside the uterine cavity and defines as cancer like model. Noscapine is a safe cough suppressant which has been introduced as cancer suppressor. The aim of this study was to investigate the in vitro effect of noscapine on human endometriotic epithelial cells. Materials and Methods: Human endometriotic biopsies (n=7) were digested by enzymatic method (collagenase, 2 mg/ml). Epithelial glands were collected by sequential filtration through nylon meshes (70 and 40 μm). The cells were divided to five groups: control and 10, 25, 50 and 100 μM concentration of noscapine and cultured for 72 hours. Viability of cells was assessed by trypan blue staining; also Nitric oxide concentration was measured by standard Griess reagent. Data was analyzed by one way ANOVA. Results: Viability of endometrial epithelial cells were 88.2, 82.0, 73.6, 72.1, and 60.7% in control, 10, 25, 50 and 100 μM noscapine concentration respectively. Nitric oxide concentrations were 57.7, 33.8, 31.4, 29.2 and 20.4 μM in control, 10, 25, 50 and 100 μM groups respectively (p<0.05). Conclusion: Noscapine decreased endometriotic epithelial cells viability in dose dependant manner. It can suggest for endometriosis treatment.